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A B C D E F G H I J K L M N O P Q

Test Identifier Information

 
Registration CodeHAPT
Method

As a stand alone HAPTOGLOBIN test

End-point Nephelometry: - Latex particles are coated with protein specific anti-human antibodies (e.g. anti-Haptoglobin) forming antigen:antibody complexes, when exposed to serum containing that specific protein. The latex particles improve sensitivity by increasing the turbidity of the antigen:antibody complex in solution. Quantitative nephelomtric measurement of the turbidity is proportional to the concentration of the protein in the serum. Serum protein concentrations can then be calculated by comparing the turbidity to that of a known standard. 

As part of the Serum Protein Profile (SPE)

Immunoelectrophoresis: - Electrophoretic separation of proteins in an anti-sera impregnated gel resulting in quantitative identification of specific proteins. 

Diagnostic Use / Indications

Haptoglobin is an

  • indicator of intravacular haemolysis as it binds to the haemoglobin released from ruptured erythrocytes
  • acute phase reactant, increasing in acute phase reactions.

 

External Price$40.48(Exclusive of GST)
  

Specimen Collection

 
Specimen Collection Protocols

Allow specimen to clot completely at room temperature.

Spin down and separate serum (or plasma) from red cells as soon as possible.

Haemolysed samples are not acceptable.

Patient SpecimenBlood 5-10 mL,Plain(Red),Heparin(Green),EDTA(Lav)
Paediatric SpecimenBlood 0.2 mL Plain(Red),Heparin(Green),EDTA(Lavender)
  

Instructions for Referral to CHLabs

 
Aliquot InstructionsMinimum 100 μL; Preferred >0.5 mL serum (preferred) or plasma; Refrigerate
Aliquot Transport to CHLAsap - on ice or ambient
  

CHLabs Laboratory

 
DepartmentBiochemistry - Protein Laboratory
Contact Details Email Email
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Contact Phone Number(03)3640334 | x80334
Test AvailabilityTuesday, Thursday and Friday. 8.00 am - 4.00 pm. Urgent testing by arrangement
Turnaround Time1 - 3 days
Reference Interval
AgeConcentration*% Normal
Newborn-6 months# 0-20
6-12 months 20-40
1-10 years 30-60
10-18 years 40-100
Adult0.6 - 3.7 g/L50-125

*Congenital anhaptoglobinaemia is common in African-Americans.

People who perform regular strenuous exercise may have sustained low levels of haptoglobin, due to low grade haemolysis.

#Neonates generally have no measurable haptoglobin.

Interpretation

Low levels of haptoglobin are suggestive of intravascular haemolysis, however haptoglobin is also an acute phase protein resulting in an elevation of levels in acute pahe reactions. This elevation may mask intravascular haemolysis, and should be monitored by the use of other acute phase proteins such as CRP or alpha 1-antitrypsin.

Sustained levels of haptoglobin may also be seen in:

  • People who do regular strenuous exercise or activities - suggesting a low grade haemolysis.
  • Patients with severe liver disease
  • Neonates
  • African-Americans

Levels should return to normal within about a week if haemolysis ceases. Therefore if tests are low, they should be repeated one to two weeks after an episode of acute haemolysis.

 

Additional Information

Haptoglobin data sheet

Refer to Biochemistry - Protein Laboratory Ext 80334

Delphic Number Test Number3218

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